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產(chǎn)品詳情

  • 產(chǎn)品名稱：1,25-Dihydroxyvitamin D3 (Calcitriol) Antibody Pair

  • 產(chǎn)品型號：abx370872
  • 產(chǎn)品廠商：abbexa
  • 產(chǎn)品文檔：
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簡單介紹：
1,25-Dihydroxyvitamin D3 (Calcitriol) Antibody Pair
詳情介紹：
Calcitriol Antibody Pair for use in Competitive ELISA assay development. This antibody pair contains:

Component 5 × 96 tests 10 × 96 tests
Capture Antibody200 μg 400 μg
Biotin-Conjugated Competitor 50 μg 100 μg
Standard 2 μg 10 μg

Please note that quantities and concentrations may change between different batches.

It is recommended to use this antibody pair with abx098959 Antibody Pair Support Kit (Competitive Method).

Target 1,25-Dihydroxyvitamin D3 (Calcitriol)
Reactivity General (All species)
Tested Applications ELISA
Recommended dilutions Dilute the Capture Antibody 125-fold with Coating Buffer.
Dilute the Biotin-Conjugated Competitor 200-fold with Biotin-Conjugated Competitor Diluent.

Optimal dilutions/concentrations should be determined by the end user.
Form Liquid (Capture Antibody and Biotin-Conjugated Competitor)
Reconstitution Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Buffer The Capture Antibody and Biotin-Conjugated Competitor both contain 0.1% sodium azide.
Test Range 123.5 pg/ml - 10000 pg/ml
Standard Form Lyophilized
Assay Type Competitive
Capture Antibody Host Rabbit
Capture Antibody Clonality Polyclonal
Capture Antibody Conjugation Unconjugated
Detection Antibody Conjugation Biotin
Concentration Capture Antibody: 0.5 mg/ml
Biotin-Conjugated Competitor: 0.2 mg/ml
Availability Shipped within 5-20 working days.
Note This product is for research use only.
Directions for use Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. Working solutions should be prepared and used immediately.

Recommended Procedure:

  • Dilute the Capture Antibody to working concentration using Coating Buffer. Immediately coat the 96-well plate with diluted Capture Antibody (100 μl per well). Seal the plate and incubate at 4 °C overnight or at 37 °C for 2 hours
  • Aspirate the wells and wash with Wash Buffer (350 μl per well) and allow to soak for 1-2 min. Remove the liquid by inverting and tapping the plate on to absorbent paper.
  • Block the plate with Blocking Buffer (200 μl per well) at 37 °C for 1.5 hours.
  • Repeat the aspiration/wash process in Step 2.
  • Add 50 μl of standards or sample into the appropriate wells, followed by 50 μl of working Biotin-Conjugated Competitor. Cover with a plate sealer and incubate at 37 °C for 1 hour.
  • Repeat the aspiration/wash process in Step 2.
  • Add appropriately diluted Streptavidin HRP (100 μl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 30 min.
  • Repeat the aspiration/wash process in Step 2, for a total of 5 times.
  • Add Substrate Solution (90 μl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 10-20 min. Keep the plate in the dark and avoid exposure to light.
  • Add Stop Solution (50 μl per well). Tap the side of the plate to ensure thorough mixing.
  • Measure the absorbance immediately using a microplate reader set at 450 nm.
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